Protein Surface Charge and Hydrophobic Patch Analysis
Protein patch analysis is a computational method for identifying and assessing protein interaction hot spots which may be implicated in aggregation, viscosity or solubility of antibodies and related derivatives. Here, we describe an enhanced and recalibrated method for calculating protein patches to better highlight potentially important interaction sites. Multiple structure support facilitates examination of interfaces and comparison across mutation series. Additionally, a new 2D patch depiction can be used in comparative analysis to accentuate patch differences and identify key active regions. Examples demonstrate how the application can be used to help understand complexation in different contexts.